| 质粒名称: | pFUGW-H1 empty vector |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 25870 |
| 存储实验室: | Sally Temple |
|
载体骨架基本信息 |
|
| 载体名称: | FUGW-H1 |
| 载体出品公司: | Ivanova, NB |
| 载体抗性: | 氨苄霉素 |
| 完整质粒大小: | 10130 bp |
| 载体类型: | 哺乳细胞表达，慢病毒载体 |
| 筛选标记: | 博莱霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | None |
| 启动子: | H1 |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | see below(未破坏) |
| 3' 克隆位点: | see below(未破坏) |
| 5' 测序引物 | H1 (5'-tcgctatgtgttctgggaaa-3') |
| 3' 测序引物 | cagtgcaggggaaagaatagtagac |
|
文献引用方法 |
|
| 材料和方法部分: | pFUGW-H1 empty vector was a gift from Sally Temple (爱迪基因 plasmid # 25870) |
| 参考文献部分: | shRNA knockdown of Bmi-1 reveals a critical role for p21-Rb pathway in NSC self-renewal during development. Fasano CA, Dimos JT, Ivanova NB, Lowry N, Lemischka IR, Temple S. Cell Stem Cell. 2007 Jun 7. 1(1):87-99. 10.1016/j.stem.2007.04.001 PubMed 18371338 |
买家导航
pFUGW-H1 empty vector载体质粒基本信息
pFUGW-H1 empty vector质粒图谱载体图谱和pFUGW-H1 empty vector载体序列质粒序列多克隆位点信息
pFUGW-H1 empty vector质粒载体简介
pFUGW-H1 empty vector质粒序列载体序列
LOCUS pFUGW-H1 empty vector 10130 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION 3rd generation lentiviral vector.
ACCESSION .
VERSION .
KEYWORDS pFUGW-H1 empty vector
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10130)
AUTHORS Fasano CA, Dimos JT, Ivanova NB, Lowry N, Lemischka IR, Temple S
TITLE shRNA knockdown of Bmi-1 reveals a critical role for p21-Rb pathway
in NSC self-renewal during development.
JOURNAL Cell Stem Cell. 2007 Jun 7. 1(1):87-99.
PUBMED 18371338
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pFUGW-H1 empty vector|
FEATURES Location/Qualifiers
source 1..10130
/organism="synthetic DNA construct"
/mol_type="other DNA"
polyA_signal 417..538
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(454..473)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 508..527
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(587..603)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(587..603)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(600..622)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 611..627
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(635..665)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 680..701
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(818..835)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(989..1577)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1069..1088)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(1748..2608)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 2371..2390
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(2609..2713)
/gene="bla"
/label=AmpR promoter
primer_bind complement(2788..2807)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 2979..3358
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 3360..3558
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 3513..3533
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 3576..3756
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 3803..3928
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 4421..4654
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 5181..5298
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter complement(5402..5616)
/label=H1 promoter
/note="human H1 RNA promoter"
primer_bind complement(5462..5481)
/label=H1
/note="Human H1 promoter, forward primer"
promoter 5645..6044
/label=UbC promoter
/note="human ubiquitin C promoter"
primer_bind 6693..6712
/label=hUBCpro-F
/note="Human Ubiquitin C (UbC) promoter, forward primer"
regulatory 6819..6828
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
primer_bind complement(6870..6891)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(7131..7150)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 7479..7500
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 7572..8160
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(7625..7645)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(8043..8054)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(8163..8179)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8164..8180)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 8685..8865
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(8882..8899)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 8888..9112
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 9158..9586
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(9245..9264)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 9455..9476
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(9595..9615)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 9600..9929
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 9780..9915
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 9842..9861
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 9977..10024
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 10043..287
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
ORIGIN
1 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
61 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
121 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
181 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
241 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
301 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
361 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
421 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
481 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
541 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
601 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
661 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
721 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
781 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
841 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
901 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
961 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
1021 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
1081 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
1141 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
1201 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
1261 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
1321 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
1381 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
1441 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
1501 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
1561 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
1621 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
1681 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
1741 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
1801 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
1861 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
1921 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
1981 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
2041 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
2101 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
2161 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
2221 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
2281 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
2341 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
2401 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
2461 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
2521 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
2581 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
2641 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
2701 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
2761 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
2821 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
2881 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
2941 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
3001 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
3061 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
3121 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
3181 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
3241 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
3301 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
3361 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
3421 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
3481 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
3541 ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct
3601 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
3661 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
3721 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
3781 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
3841 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
3901 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
3961 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
4021 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
4081 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
4141 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
4201 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
4261 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
4321 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
4381 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
4441 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
4501 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
4561 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
4621 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
4681 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
4741 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
4801 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
4861 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
4921 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
4981 tttgtatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
5041 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
5101 gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa
5161 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
5221 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
5281 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggttaatt
5341 aagaaaggat ccgaaagaat tcgcccttag atctctagag gagctttctt cccgggaaag
5401 agtggtctca tacagaactt ataagattcc caaatccaaa gacatttcac gtttatggtg
5461 atttcccaga acacatagcg acatgcaaat attgcagggc gccactcccc tgtccctcac
5521 agccatcttc ctgccagggc gcacgcgcgc tgggtgttcc cgcctagtga cactgggccc
5581 gcgattcctt ggagcgggtt gatgacgtca gcgttcttaa ttaacccgtg tcggctccag
5641 atctggcctc cgcgccgggt tttggcgcct cccgcgggcg cccccctcct cacggcgagc
5701 gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg
5761 acagcggccc gctgctcata agactcggcc ttagaacccc agtatcagca gaaggacatt
5821 ttaggacggg acttgggtga ctctagggca ctggttttct ttccagagag cggaacaggc
5881 gaggaaaagt agtcccttct cggcgattct gcggagggat ctccgtgggg cggtgaacgc
5941 cgatgattat ataaggacgc gccgggtgtg gcacagctag ttccgtcgca gccgggattt
6001 gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag cgggctgctg
6061 ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg tgtggagaga
6121 ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg ggttgggggg
6181 agcgcagcaa aatggcggct gttcccgagt cttgaatgga agacgcttgt gaggcgggct
6241 gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc aagaacccaa ggtcttgagg
6301 ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg gctggggcac catctgggga
6361 ccctgacgtg aagtttgtca ctgactggag aactcggttt gtcgtctgtt gcgggggcgg
6421 cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg
6481 tgtcgtgacg tnncccgttc tgttggctta taatgcaggg tggggccacc tgccggtagg
6541 tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg ggcctagggt aggctctcct
6601 gaatcgacag gcgccggacc tctggtgagg ggagggataa gtgaggcgtc agtttctttg
6661 gtcggtttta tgtacctatc ttcttaagta gctgaagctc cggttttgaa ctatgcgctc
6721 ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg
6781 gtcaatatgt aattttcagt gttagactag taccggtcgc caccatggtg agcaagggcg
6841 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
6901 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
6961 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
7021 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
7081 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
7141 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
7201 tgaagggcat cgacttcaag gtaggacggc aacatcctgg ggcacaagct ggagtacaac
7261 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
7321 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
7381 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
7441 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
7501 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcaa ttcgatatca
7561 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
7621 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
7681 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
7741 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
7801 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
7861 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
7921 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
7981 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
8041 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
8101 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
8161 atcgataccg tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag
8221 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
8281 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
8341 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata
8401 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac
8461 cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
8521 agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga
8581 gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc
8641 tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag
8701 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
8761 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
8821 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagaaac ccgctgatca
8881 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
8941 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
9001 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
9061 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
9121 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
9181 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
9241 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
9301 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
9361 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
9421 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
9481 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
9541 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
9601 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
9661 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
9721 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
9781 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
9841 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
9901 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
9961 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
10021 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
10081 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
//
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